Intermediate
Part:BBa_K208030:Design
Designed by: USU iGEM 2009 Group: iGEM09_Utah_State (2009-10-20)
GFP/HlyA/Terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 4
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 523
Illegal XhoI site found at 424 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Silver Fusion-friendly. GFP, HlyA secretion tag, and two terminators in pSB1AK3 vector. Very effective GFP, attach to C-terminus.
Source
Composite