Intermediate

Part:BBa_K208030:Design

Designed by: USU iGEM 2009   Group: iGEM09_Utah_State   (2009-10-20)

GFP/HlyA/Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 523
    Illegal XhoI site found at 424
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Silver Fusion-friendly. GFP, HlyA secretion tag, and two terminators in pSB1AK3 vector. Very effective GFP, attach to C-terminus.

Source

Composite

References